Can anybody help me checking cytotoxicity of compounds in human macrophages (not cancerous)

20 October 2011 0 9K Report

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How to create Residue Topology File in CHARMM?

Hello All Can anyone help me with the simple tutorial(other than the common one available on the web) to construct a residue topology file for bimolecular interaction (Vander waal) in CHARMM....

24 February 2016 2,027 12 View

What are informatics tool to identify a new specific gene for conformation of micro organism?

Hi all I am working on food borne pathogens. now I want to design a PCR based techniques for the conformation of a specific organism. I know that, already there are so many genes in literature for...

22 April 2015 6,059 2 View

Can any one suggest the modelling, docking softwares other than Modeller anfd autodock?

i have alrady tried modeller 9.10 for modelling, but model generated from Modeller had large threads but ramchandran plot gives 97% allowed regions. can it be used for docking studies or not?

27 August 2012 728 0 View

I am facing problem in growing LNCap androgen sensitive prostate cells.Cells are not adhering to flask. Can anyone suggest me reg.this

I am growing LNCaP cell sfrom past 10 days . cells look round and healthy but not adhering to flask. Can any one help me in this reg

10 January 2012 1,603 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View