Can anybody help me checking cytotoxicity of compounds in human macrophages (not cancerous)

20 October 2011 0 9K Report

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Cell death post transduction and treatment with puromycin?

I am trying to knockdown a protein in Jurkat cells by shRNA lentiviral transduction. I prepare virus particles in HEK-293T cells using shRNA construct + psPAX2 + pMD2.G in the ratio given on...

12 December 2021 1,105 5 View

How to fix an image after pymca batch processing?

after completing the batch processing of my data in pymca my 3D image has become 2D and splitted into half.how do i merge left half of the image into right half and bring it back to 3D? (image on...

16 August 2021 7,437 3 View

How to create Residue Topology File in CHARMM?

Hello All Can anyone help me with the simple tutorial(other than the common one available on the web) to construct a residue topology file for bimolecular interaction (Vander waal) in CHARMM....

24 February 2016 2,175 12 View

Is there an expression strain that yields in low endotoxin proteins?

Obtaining an endotoxin free protein is quite a challenging task., Although it can be achieved, is there an E.coli strain that is LPS free?

17 November 2015 488 3 View

How can I remove the rhfpro error in Molpro?

Hello I am new to the computational suite"MolPro" and I am trying to do the frequency calculation for a small organic molecule. Following is my Input data: ***, Name frequency...

31 August 2015 7,356 6 View

How can I calculate the MPE and energy density for 281nm wavelength, 10hz 6ns pulsed laser output of 200 micro joule?

wavelength =281nm laser spot=10mm energy used = 200uJ/pulse laser specifications 10hz, 6ns , pulsed

23 August 2015 8,134 3 View

What are informatics tool to identify a new specific gene for conformation of micro organism?

Hi all I am working on food borne pathogens. now I want to design a PCR based techniques for the conformation of a specific organism. I know that, already there are so many genes in literature for...

22 April 2015 6,125 2 View

Can any one suggest the modelling, docking softwares other than Modeller anfd autodock?

i have alrady tried modeller 9.10 for modelling, but model generated from Modeller had large threads but ramchandran plot gives 97% allowed regions. can it be used for docking studies or not?

27 August 2012 816 0 View

I am facing problem in growing LNCap androgen sensitive prostate cells.Cells are not adhering to flask. Can anyone suggest me reg.this

I am growing LNCaP cell sfrom past 10 days . cells look round and healthy but not adhering to flask. Can any one help me in this reg

10 January 2012 1,685 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View

How can I put an alert to receive notifications of call for papers in my research field?

I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...

01 June 2024 1,352 0 View

How many percent of specific antibodies in serum after rabbit immunization ?

Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...

13 May 2024 2,828 0 View

What role do autoimmune disorders play in causing infertility?

how can immunological interventions potentially address these issues?

04 April 2024 4,951 1 View

PhD candidate with a research focus on chronic inflammation particularly macrophage cells so i need professor information this area??

I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...

28 March 2024 7,798 0 View

How can I add weight to difference in correlation between 2 groups to also reflect the higher correlation values in addition to the difference?

I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...

11 March 2024 1,000 4 View