Can anybody give buffers composition that is more closely resemble intracellular conditions of fungal cell.

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kindly reply me. Thanking you in advance.

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Steingrimur Stefansson

Hi, intracellular conditions of eukaryotic cells are nearly identical, so you can use generic pull down assays.

Aamal Ghazi Mahdi Al-Saadi

That is true Steingrimur

Dharmendra Nath Bhatt

Thank you for your valuable suggestion Steingrimur Stefansson and Aamal Ghazi Mahdi Al-Saadi..