I am working on endophytic fungi and i want to test minimum inhibitory concentration for my endophytic fungal extracts so any body explain the simpler procedure for me.
Hello Nischitha,
As Bedreddine pointed out above, this procedure is pretty easy.
Essentially, you do serial dilutions of what you wish to test and see at what concentration your OD600 (mostly) returns to a no bacteria/fungi mark.
As a consideration, remember to make sure you have no particulate in the wells, especially at the higher concentrations. Even if everything is solubilized when you plate the bacteria/fungi and the compound, high concentrations can crash out of solution. This can cause light scattering and false results. You'll know you have this when your curve seems to kill the bacteria, but then the OD raises at a higher concentration with bad margins of error. If you would like, I can send you some examples of what this looks like.
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