How to prepare a Gaussian input file for a binary solvent?

The UV absorption energy spectrum of a molecule has been recorded experimentally using binary solvent (CH3Cl:CH3OH =10:1 v/v). I am trying to calculate the absorption energy of the same molecule...

15 March 2020 9,396 3 View

How can i track a mining area through satellite imagery (Sentinel 2 for example) ?

I'm trying to visualize the changer in a mining area over time (the new buildings, the exploitation ..) and I can't find enough documentation, I prefer to do this work in python since I'm familiar...

15 January 2020 373 7 View

Extraction of fish protein?

Simple method for extractiom of protein fish

06 October 2019 4,429 3 View

Does anyone have experience with DNA extraction from formalin fixed paraffin embedded (FFPE) tissue?

I am using QIAamp DNA FFPE Tissue Kit for DNA extraction; i followed the manufacturer protocol but i've got a smeer, what should i do. thank you for your help

05 February 2018 3,207 4 View

How we can do correction of abnormal pixels in Hyperion images using ENVI or ERDAS softwares ?

How we can do corrections of abnormal pixels in Hyperion images using ENVI or ERDAS softwares ? We do it before geometric corrections ? Before classification ? or we can do it after...

04 February 2018 7,381 2 View

How do I perform atmospheric correction in EO-1 Hyperion data? how i can remove the noisy bands and eliminate also the bad line ?

I need to remove the noisy bands of Hyperion EO1 image , i downoald the image from earthexplorer.usgs.gov as 242 seperate bands. and what is the good method in ENVI 5.3 for doing athmospheric...

28 January 2018 9,454 3 View

What is the best LPS-dose to induce chronic systemic inflammation to mice ?

Hi, I am starting a project that requires to induce a chronic systemic inflammation to mice. I try to figure out which dose of LPS I can give to mice and maybe how many times I should give them to...

21 January 2018 3,648 6 View

Resampling remote sensing image for image fusion ?

i have a hyperspectral image with 3m spatial resolution and have multispectral image with 1.6 m spatial resolution. I want to resample the hyperspectral image resolution to 6.4 m after i should...

18 January 2018 1,797 15 View

What is the effect of organic solvents on DNA conformation?

does the strucure of DNA will be conserved in organic medium?

31 October 2017 4,974 5 View

Matching between insilico PCR product size and agarose gel resultas after PCR reaction?

31 January 2017 6,987 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View