Can any one simplify method of TTC staining of the brain to determine infarction volume ?

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Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

I need a digital Transformation validated measurement scale ?

Thanks for support

25 June 2024 4,475 0 View

Trying to make optimization for Mixer on different pdk ?

i make a design topology of mixer For my graduation project working at 28 Ghz on cadence with UMC pdk and get this result S(2,1) 3.5db but when trying to make same topology with another pdk on...

09 June 2024 9,729 3 View

How can i remove the formed water in schiff base synthesis to shift the equilibrium towards the imine?

I am trying to form some ligands and some time it necessary to remove the water form by the elimination of it from the reaction .

10 May 2024 8,899 5 View

Could you email me this paper below?

I'm interested in this paper : A Data Dissemination Algorithm Based On Maximization of Causal Path Entropy in Vehicular Ad Hoc Networks Could you email me this paper?

19 April 2024 2,940 0 View

Hello, very good day everyone. I wanted to ask how many rpm to obtain the separation of a drug sample in wastewater with activated sludge?

I need to separate my drug from a sample with activated sludge and residual water

17 April 2024 9,601 2 View

How to characterize the biogenic nanoparticles for the capping agents? How can capping agents be isolated ? Can anyone please share an article ?

Seeking a protocol to isolate the capping agents from prepared nanoparticles how to characterize them ?

01 April 2024 6,513 3 View

Why my unstained, isotype (IgG) and positive samples (U87 cells) have same histogram results?

Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...

23 March 2024 8,883 1 View

How can i complete clustering analysis and FEL analysis using gromacs ?

GROMACS version: 21.0 GROMACS modification: No I complete a 100 ns MD using gromacs v21.0 Can you please provide me a step by step command lines to complete clustering analysis and Free energy...

03 March 2024 2,482 1 View

Title: Measuring Zeta Potential of Nanosheets with Unknown Refractive Index and Handling High Polydispersity Index in Gel-Based Nanosheets ?

I am currently working on measuring the zeta potential of nanosheets, but I've encountered a challenge related to the unknown refractive index (RI) of my samples. Since the exact RI of the...

20 February 2024 2,420 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View