I have tried it for doing a western blot- 1 tube with SDS lysis buffer (Laemelli Buffer with standard dyes and DTT) and second tube only using boiling in a simple TRIS buffer (heating block at 95C, 15 mins). The protein obtained from tube 1 successfully gave bands in westerns but I did not get any signal from tube 2. My guess was that they did not lyse well. Adding 1% SDS to tube two helped. If you are trying to make RNA, TRIZOL/any RNA extraction kit buffers help. I have not checked the extent of lysis by any imaging method though.