I want to grow plant virus in laboratory conditions.So can any one suggest me which media will be better to grow plant virus? Can any one suggest me regarding standard condition for plant virus culture ?
Dear Manasa Kumar Panda
Pls. find the attached files
http://www.sciencedirect.com/science/article/pii/S0168165609003034
http://www.cabdirect.org/abstracts/20001004538.html;jsessionid=BDBFBB0B8114C198529309FBAF0DACFE
http://www.microbiologyinfo.com/techniques-of-virus-cultivation/
http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.1953.tb30257.x/abstract
http://www.ncbi.nlm.nih.gov/pubmed/19616595
http://link.springer.com/chapter/10.1007/978-3-642-68528-6_3#page-1
Curtis, Jeanette; Caroline Rea (25 May 2007). "Viral culture". WebMD. Retrieved 2009-09-09.
Storch, Gregory A.; Bernard N. Fields; David Mahan Knipe; Peter M. Howley (2007). "Diagnostic virology". In David Mahan Knipe, Peter M. Howley. Fields' Virology 1 (5th ed.). Lippincott Williams & Wilkins. p. 3177. ISBN 0-7817-6060-7.
Table 2 in: Leland DS, Ginocchio CC (January 2007). "Role of cell culture for virus detection in the age of technology". Clin. Microbiol. Rev. 20 (1): 49–78. doi:10.1128/CMR.00002-06. PMC 1797634. PMID 17223623.
Viral Culture, General". LabCorp. Retrieved 2009-09-09.
"Growing viruses from clinical samples.". London Laboratory Services Group. Retrieved 2009-09-09.
Regards
Prof. Houda Kawas
What is your purpose for growing plant viruses in culture? Is it for assays? If you want to produce more virus (increase inoculum) for producing antibodies or for virus characterization studies, you can usually inoculate specific plant species (live plants such as certain tobacco varieties etc., depending on the virus) that are known to produce high titers of the particular virus of interest, and then purify the virus in greater quantities and more efficiently from inoculated plant tissue than from plant tissue culture.
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