Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How can we make other Machine Learning Algorithms Deep like Deep Neural Networks?

For example Decision Tree, Ant Colony Optimization etc.

26 December 2020 6,804 3 View

Are there any Uni-Dimensional scales available for social media motivation?? Or simply motivations to use social media in context of luxury brand?

I'm looking for Uni-dimensional scales for measuring motivation towards using social media. Or simply social media motivation. In context of social media marketing and Luxury fashion Brand's brand...

13 December 2020 4,929 3 View

How adsorbed Mercury (II) react with HCl and HNO3 in water?

Case: Hg2+ is adsorbed on a titanium carbide and 0.1 M HCl/HNO3 is used for the desorption of Hg2+. During the reaction, the solution turns into a pale yellow color. I want to understand what...

16 November 2020 5,770 3 View

Are there any Uni-Dimensional item Scales available for Social Media Marketing? kindly recommend me articles or item scales for Uni-dimensional SSM ?

I couldn't find Social media marketing uni dimensional item scales. Kindly recommend or share with me articles having only uni dimensional measurement scales of Social media marketing to check its...

09 November 2020 8,793 3 View

How to link excel with Aspen plus?

Is there any way to connect excel data sheets with aspen plus as inputs to perform parametric analysis?? I want to change input parameters for a certain range to see system performance

28 October 2020 2,174 3 View

Help me about swelling behavior of graphene oxide nanocomposites with methyl methacrylate with different proportions of GO?

Swelling behaviour of PMMA with GO contents 0.1%, 0.2,0.3,0.5?

02 October 2020 2,655 3 View

Can all network UE decode the information transmitted by a eNB through Physical Downlink Control Channel (PDCCH)?

A wireless network consisting of two UEs (A & B) and an eNB. The eNB transmits control information to UE A through PDCCH. Can UE B also decode this information? Or is it permissible for UE B...

07 August 2020 2,628 2 View

Any useful softwares for applying neural networks in power systems?

Any useful software for applying neural networks in power systems? I know MATLAB has a NN toolbox but besides that, is there any other useful software available?

20 April 2020 9,663 16 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View