Can any one give me a protocol for beta gal ONPG assay for AML12 transfected cells?

31 October 2017 1 2K Report

I have a protocol which we used a few years ago with 3T3 cells. With the AML12 cells I don't get any color. Any suggestions?

Thank you.

Flavie Sicard

Sometimes, beta-gal expression is too low to be detected by ONPG assay, you have to proceed to a more sensitive assay like CPRG.

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