The dtection of known Food allergen from food matrix and its antibody binding is a long way and approach require a lot of batch process and experiment. These days several method is coming according to different prodcut or matrix homogenous or heterogenous food matrix. My question is for homogenous food, one food matrix have also various allergenic protein and identify all of them together is not so easy with efficiency. I would like to get some expert comments and suggestion to apply the best way to detect maximum allergen together and its antigen binding efficiency and if possible then modification in technique?
Thank You all
You are right. This question is not easy. Since most of the different allergens of a specific food are not structurally related, the complete detection seems to be not possible. You may either focus on the most frequent allergens or on the dominant or most stable protein in the food. Please also consider the use of PCR, which detects food on the basis of their nucleic acids, not proteins.
Hi,
If I correctly understood your question, a method of "Component resovled diagnostics" may be useful. It permits detect mayor and minor allergens. As to peanut allergens, there are :
Ara h 2 - nsLTP
Ara h 5 - profilin
Ara h 8 – PR-10 group
Ara h 1, Ara h 2, Ara h 3, Ara h 6, Ara h 7 – storage allergens.
All of them may be detected using ImmunoCAP, or ImmunoCAP-ISAC methods, depending on the quantity of allergens.
Best wishes!
- Badges
- Science topic
- Similar topics
- Medicine
- Nutrition and Dietetics
- Nutritional Medicine
- Food Science
- Food