immunological study.
Regarding diabetes treatment study
05 June 2017 6,971 3 View
Study of microbiology
11 December 2016 1,999 0 View
Study of Bacteria
11 December 2016 1,949 5 View
relating to cell death
05 June 2016 9,864 13 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.
26 July 2024 2,702 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?
20 July 2024 4,020 0 View
How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?
19 July 2024 5,239 2 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View