Hello, the lipids have to be dried under vacuum to form a film (preivously lipids should be completely dissolved in organic solvents). Then the film can be hydrated with water to form liposomes. Sometime you need higher temperature to hydrate the film properly.When you reach and omogenous solution, you have to pass it into a filter to obtain liposomes of the appropriate size you require.

Of course liposomes will form if you use fosfolipids... not just random polymers.

Regards

Hi Rumpa,

You can make liposomes without using toxic solvents, e.g. by employing "Mozafari method".

I am attaching some publications explaining this safe and robust procedure. Let me know if you need details.

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Prof. M. R. Mozafari,

Australasian Nanoscience and Nanotechnology Initiative (ANNI),

8054 Monash University LPO, Clayton, Victoria 3168, Australia.

http://anni.com.au/

Emails: [email protected] & [email protected]

Tel: +61 3 87538268 and +61 424339961

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I may suggest you to go through the below-mentioned two review articles, which will give you the enough information on liposomes and their preparation methods. The second articles will explain the modified and new methods for liposome preparation.

1. Akbarzadeh et al., 2013. Liposome: classification, preparation, and applications. Nanoscale Res Lett. 8, 102. DOI: 10.1186/1556-276X-8-102.

2. Yogita P.Patil, SameerJadhav, 2014. Novel methods for liposome preparation. Chemistry and Physics of Lipids, 177, 8-18. DOI: 10.1016/j.chemphyslip.2013.10.011.