Hello everyone, I am performing ChIP in plant tissues. I bought a ChIP grade antibody from a company but it came in serum format (unpurified). My question is, do I need to purify it first or do I use it in the same way that a purified antibody is used in ChIP? and is there any performance difference between purified and unpurified antibodies?
Thank you very much
Yoav Lubelsky
I have successfully used un-purified antibody for ChIP in the past so it is possible, at the end of the day it depend on how good your antibody is. I suggest you do a small pilot ChIP-qPCR type experiment and see if your AB is good enough as is, if not you can try purifying it.
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