Valentina Casella

3 Questions 2 Answers 0 Followers

Questions related from Valentina Casella

Can an insoluble compound be absorbed if injected intraperitoneally in mouse?

We want to study immune responses in mice treated with a lipophilic compound that has to be dissolved in DMSO. Given the toxicity and potential effect on immune response due to administration of...

15 June 2023 4,843 2 View

How to quantify RNA copies using cDNA standard curve in RTqPCR?

I have LCMV RNA samples of unknown concentration and I want to know how many LCMV RNA copies/mL I have in each sample. To do so, I am going to use the same volume of RNA/sample to perform a One...

02 May 2022 8,959 3 View

How long can sorted cells be kept in RLT buffer+ 2B-mercaptoethanol on ice, before proceeding with RNA extraction?

Hi! I need to extract RNA from sorted immune cells using RNeasy Quiagen minikit , and having many samples I would process them approximately 2 hours after being sorted. Because of this delay, I...

11 May 2018 8,086 5 View