Can alpha glucosidase and alpha amylase work at room temperature and at 37*C? If so why? The specifications for these enzymes are that they work at 37*C. But number of papers have modified their protocols 25*C. How can it work at both room temperature and at 37*C?
My enzyme assay at 37 C is not coming out well. There is no variation between blank and the test. But at 25 C there is difference. I don't understand the logic behind this. Enzymes are suppose to work at 37 C right?
All enzymes can, the only difference is in the rate of catalysis at different temperatures.
Dear Indumati,
every enzyme have its optimum pH and optimum temperature. At its optimum pH and optimum temperature the enzyme efficiency is maximum but enzyme do not stop catalysis completely below and above the optimum pH or temperature but work at a different rate.
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