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Questions related from Indumati Sharma
I have an lenti-transfer vector encoding puromycin resistant gene driven by PGK promoter. I need to select puromycin resistant clones after LV transduction. What is the best way I can select...
14 June 2019 2,981 1 View
I want to know which one is more suitable for dendritic cell transduction. I see that in: https://www.cell.com/cell/pdfExtended/S0092-8674(15)00829-6 PAX2 plasmid is used for dendritic cells...
20 January 2019 2,815 3 View
When publishing a research paper, ideally, how many authors should there be in a paper? Does having too many authors impact the credibility of the first author? Does having few authors (like two...
20 September 2013 2,855 10 View
I'm running TLC with silica sheets. As the solvent travels quarter way up its movement changes such that we no longer see a straight line of solvent but rather a squiggly line. This largely...
12 September 2013 8,455 14 View
What should be the nutritional status of wistar rats before the injection of STZ.? I came across this very interesting paper titled 'Streptozotocin is equally diabetogenic whether administered to...
11 August 2013 3,170 1 View
Are reheated foods unhealthy in the long run? Eg. Reheating rice items, curry, rasam etc? Number of doctors are of the opinion that reheated foods are unhealthy. But they are unable to...
04 August 2013 9,436 15 View
Can we measure antioxidant status, like CAT, SOD, GSH a week after autopsy of wister rats? I know that LPO should be measured the day of autopsy. But what about other parameters? Is it true that...
16 July 2013 1,554 0 View
In Alpha glucosidase assay does PNPG (from SRL) degrade readily if kept at room temperature for about 2-3h?
22 April 2013 8,711 1 View
Can alpha glucosidase and alpha amylase work at room temperature and at 37*C? If so why? The specifications for these enzymes are that they work at 37*C. But number of papers have modified their...
16 April 2013 3,464 3 View
Anyone have a protocol for the lipid peroxidase enzyme assay invitro? I need to check my plant's inhibitory activity towards lipid peroxidation.
13 March 2013 8,956 3 View
For DPPH, total antioxidants and lipid peroxidation assay which control to use: Gallic acid or Ascorbic acid?
13 March 2013 888 23 View
What are the key features to look out for when inducing type 2 diabetic animal models? How will there histological and biochemical parameters be different to that of type 1 diabetic animal models?...
06 March 2013 5,423 8 View
I want to estimate GLUT4 antibody in my animal models. According to a number of publications western blot is the primary method of estimating GLUT4. But is ELISA an option? Why is Western Blot the...
14 February 2013 2,372 10 View
