The Agrobacterium Vir genes can be induced by using Acetosyringone (30-100 uM) at  the pH 5-6 before the inoculation/co-cultivation of bacteria on plants to increase transformation efficiency. During the co-cultivation also you can use it in MS media. But its waste to use Acetosyringone at pH around 7 in LB or any other media.

Acetosyringone is a flavonoid that induce the Agrobacterium vir-Genes, and its use could enhance your transformation efficiency. But your transformation efficiency also depends in the technique that you were using. If you use floral deep, your Agrobacterium culture is centrifuged to remove the LB media and resuspended in MS 0.5x with Acetosyringone 50 uM prior to inoculation, your transformation efficiency could be increased.

Regards.

yes, acetosyringone increased the transformation efficiency through agrobacterium in MS media and others I used in different cereal crops 

I too agree with the others that acetosyringone increases transformation frequency. In all our transformation experiments we are routinely employing acetosyringone in MS medium. Regarding the concentration , I feel you have to standardise it for your plant/crop. Wish you good luck

We used 100 mkM acetosyringone at pH 5.5 for Agrobacterium vir-genes induction. For transient expression we add it to bacteria resuspended in infiltration buffer  1-2 h before infiltration, for co-cultivation it can be added into MS medium.  Additionally acetosyringone might be added during bacteria growing into LB medium. To our knowledge, at least it does not decrease transformation efficiency.

We use 0.5 M stock solution in DMSO, you can keep it at +4C.

Thank you all for your valuable answers..

Absolutely it is a semisolid flavonoid belonging to phenol compounds

That initiate the signal transduction and activate the virulence gene for the transfer of TDNA 

It act as an important parameter for increasing the transformation efficiency

During agroinfiltration or co-cultivation of Agrobacterium with fresh tobacco explants it can be stimulated with wound-mediated exuded phenolic compounds. Apparently, in some cases their effect can overlap the effect of acetosyringone. However their dosage could not be exactly calculated and predicted - it depends on experiment accuracy, explant type etc. Thus, people add acetosyringone to ensure induction of vir-genes.  

yes. ACS concentration should be 150 mM.

Acetosyringone can effectively increase transformation of efficiency in plants. it has been previously reported by different scientists. "EFFECT OF ACETOSYRINGONE ON Agrobacterium-MEDIATED

TRANSFORMATION OF COTTON" can help you in this regard.

Surly it is important in signalling secreted by wounded plant and helps in increasing the transformation efficiency.

I use 200 uM in tomato transformation and works very well for me