Hi,
I am trying to measure ALDH activity in dendritic cells from intestinal lamina propria, and need to use a bunch of other antibodies to identify dendritic cells. This means I need to set up a compensation panel with Abc compensation beads, in which I do not think aldefluor kit will be able to conjugate with beads. Can I use another antibody-conjugated to FITC (i.e instead of adldefluor) to set up compensation for FL1 (FITC) channel?
Thanks
Hongbing Yu
Hi John,
Thanks a lot! I guess I will try to find fluorochromes that have minimum spillover to each other but good enough to identify dendritic cell popoulations in this assay.
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