In the pCAMBIA vector 1391z, the cassette includes a plant promoter placed upstream to GUS. Post transient expression analysis, should we expect changes in the levels of metabolites and endogenous gene expression levels because of this promoter elements?
In simple terms, can transient expression of GUS driven by plant gene promoter elements bring any change in endogenous metabolites and genes?
Katie A Burnette
The insertion of this cassette into the plant genome WILL cause mutations at the insertion site. You will need to analyze several independent insertional lines to make sure you are don't have any significant impacts from these insertion site mutations.
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