You seed same number of cell into 96-well plates, treat with nanoparticles and incubate for 24-48 hours. Is it correct to observe more cell proliferation % with the 48 hour treated cells compared to the 24 hours. Treatment is the same.
I would use time course. for example, you can do 24, 48 and 72 hours respectively
If nanoparticles is not toxic to cells, they will proliferate and show more viability than 24 hours treatment.
It happened quite often also in my experiments when the concentration of the drug used is sublityc: only few cells die while the others keep proliferating. Probably you used an amount of nanoparticles not toxic to the cells so just a little part die while the rest will proliferate
I have a similar doubt.... I'm testing 3 different incubation times: 24h, 48h and 72h.
For 24h incubation, viability starts at 100% and maintain 100% even in higher concentrations, which means it has no toxicity effect on my cell.
For 48h incubation, viability starts and maintains at 60%. And, at 72% incubation starts at 20% and with the increasing of concentration the viability increases until 100% too. Can you explain me these results?
Hi Carolina, I would suggest at 24 h your material is still not taken up by the cells hence 100 % viability, confirmed as the viability is lowered with increase in incubation time (uptake of the material is observed after 48 h). The 72 h observation is a bit strange, viability increases to 100 % implying non-toxicity of the material.
I am testing 2 different incubation times: 24 hr, 48 hr. I found that at 24 hr more survival of cells was there in comparison to 48 hr.
@Rutu Patel its possible at 24 hrs the cells are not showing complete or full influence of the treatment. Yet at 48 hours the treatment effect is more pronounced, uptake into cells is attained compared to 24 hours. Trend looks good.
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