Carolina Pereira

3 Questions 4 Answers 0 Followers

Questions related from Carolina Pereira

What is the best stimulation to detect IL-10 and IL-6 on Caco-2 by ELISA?

Does anyone know which stimulus can i use to detect Il-10 and IL-6 on Caco-2? I have cultured Caco-2 for 21 days in a 12-well plate and did the stimulation with (25 ng/mL IL1b, 10 ug/mL LPS, 50...

06 September 2018 1,428 2 View

Can incubation time influence in cell assays?

I'm testing 3 different incubation times: 24h, 48h and 72h. For 24h incubation, viability starts at 100% and maintain 100% even in higher concentrations, which means it has no toxicity effect on...

27 February 2016 3,824 5 View

How can I overcome solubility problems with Polymethoxyflavones?

I'm testing polymethoxyflavones in cell assays (Nobiletin/Tangeretin/Sinensetin) and in higher concentration tested, these PMF's showed crystallization. The stock solution are diluted in DMSO. Can...

27 February 2016 9,914 1 View