Repositories for raw data?

What are the best repositories fow my raw research data? Some journals are now requiring a DOI or an external link to the bulk data; where's the ideal place to do so (biomedical sciences)?

11 December 2017 492 4 View

Does anyone here works with ellagic acid? Could you provide me with your main literature?

Original papers and a little about your research, I'm collaborating with a group and need to read some of the literature, maybe cite it too.

10 November 2017 7,201 0 View

No title

10 November 2017 9,436 2 View

Is anyone interested in collaborating with us in a review article on Tribulus terrestris and physical activity?

We are looking for a coworker (preferably a plant biologist, nutritionist or food scientist) to help expanding an ongoing review on Tribulus terrestris and physical activity. Provisory title:...

10 November 2017 7,438 2 View

What's the best platform for pre-print publication?

BioRxiv; PeerJ; Preprints... Where can I have the best visibility for an article?

09 October 2017 8,360 2 View

What's the best method/protocol for immunofluorescence in cell cultures?

By which method you have reached an ideal/clearer staining?

09 October 2017 2,292 8 View

What are the most reliable assays to measure intracellular ascorbic acid content and release?

We aim to evaluate this in avian and mouse retina/cortex cultures.

09 October 2017 4,783 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Please, How can I plot a channel capacity of MIMO and SISO in matlab?

CSISO= f(frequency) : plot(f, CSISO) ? CMIMO= f(frequency) : plot (f, CMIMO) ?

13 February 2021 5,225 4 View