Repositories for raw data?

What are the best repositories fow my raw research data? Some journals are now requiring a DOI or an external link to the bulk data; where's the ideal place to do so (biomedical sciences)?

11 December 2017 492 4 View

Ca2+ channel blockers?

What are the best options for Ca2+ channel blockers? I need a long lasting effect (~2h) on membrane Ca2+ channels to perform some experiments with intracellular Ca2+.

11 December 2017 2,638 4 View

Does anyone here works with ellagic acid? Could you provide me with your main literature?

Original papers and a little about your research, I'm collaborating with a group and need to read some of the literature, maybe cite it too.

10 November 2017 7,201 0 View

No title

10 November 2017 9,436 2 View

Is anyone interested in collaborating with us in a review article on Tribulus terrestris and physical activity?

We are looking for a coworker (preferably a plant biologist, nutritionist or food scientist) to help expanding an ongoing review on Tribulus terrestris and physical activity. Provisory title:...

10 November 2017 7,438 2 View

What's the best platform for pre-print publication?

BioRxiv; PeerJ; Preprints... Where can I have the best visibility for an article?

09 October 2017 8,360 2 View

What's the best method/protocol for immunofluorescence in cell cultures?

By which method you have reached an ideal/clearer staining?

09 October 2017 2,292 8 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View