Hércules Rezende Freitas

8 Questions 19 Answers 0 Followers

Questions related from Hércules Rezende Freitas

Repositories for raw data?

What are the best repositories fow my raw research data? Some journals are now requiring a DOI or an external link to the bulk data; where's the ideal place to do so (biomedical sciences)?

12 December 2017 555 4 View

Ca2+ channel blockers?

What are the best options for Ca2+ channel blockers? I need a long lasting effect (~2h) on membrane Ca2+ channels to perform some experiments with intracellular Ca2+.

12 December 2017 2,715 4 View

Is anyone interested in collaborating with us in a review article on Tribulus terrestris and physical activity?

We are looking for a coworker (preferably a plant biologist, nutritionist or food scientist) to help expanding an ongoing review on Tribulus terrestris and physical activity. Provisory title:...

11 November 2017 7,500 2 View

How's viability for frozen (-80ºC) rat brain tissue in cell culture?

I'm planning to use frozen (-80ºC) rat brain tissue for cell culture purposes, will I obtain good viability? Can I culture these cells using supplemented DMEM-F12 (with FBS and B27)?

11 November 2017 9,502 2 View

Does anyone here works with ellagic acid? Could you provide me with your main literature?

Original papers and a little about your research, I'm collaborating with a group and need to read some of the literature, maybe cite it too.

11 November 2017 7,262 0 View

What's the best platform for pre-print publication?

BioRxiv; PeerJ; Preprints... Where can I have the best visibility for an article?

10 October 2017 8,421 2 View

What's the best method/protocol for immunofluorescence in cell cultures?

By which method you have reached an ideal/clearer staining?

10 October 2017 2,361 8 View

What are the most reliable assays to measure intracellular ascorbic acid content and release?

We aim to evaluate this in avian and mouse retina/cortex cultures.

10 October 2017 4,848 0 View