I'm trying to model muscle atrophy in vitro in C2C12 cells that have been differentiated for 7 days by high glucose DMEM with 2% HS and 1% Pen/Strep. I was just wondering if I use high glucose DMEM with no serum at all for 48 hours if it will induce atrophy? I read a similar paper using this method and was just wondering if it is a good model to use?
Dear Joseph, atrophy in C2C12-derived Myotubes can be also induced by treating them with 1 uM dexamethasone for 24/48 hrs. Such approach is highly reliable.
good luck
Hi Joseph,
Alessio is right. I recommend you to read the following articles:
Massaccesi L, Goi G, Tringali C, Barassi A, Venerando B, Papini N. Dexamethasone-induced skeletal muscle atrophy increases O-GlcNAcylation in C2C12 cells. J Cell Biochem. 2016;117:1833–42.
Bodine SC, Baehr LM. Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1. Am J Physiol Endocrinol Metab. 2014;307:E469–84.
Contreras, O., Villarreal, M. & Brandan, E. Nilotinib impairs skeletal myogenesis by increasing myoblast proliferation. Skeletal Muscle 8, 5 (2018). https://doi.org/10.1186/s13395-018-0150-5
*Starting at day 6 of C2C12 skeletal muscle differentiation 5uM treatment of dexamethasone induces myotubes atrophy and increases Murf1 and Atrogin-1.
Best luck,
Osvaldo Contreras
Alessio Reggio
For treatment of C2C12 cells with Dexamethasone, Serum free medium was used or 10% FBS was used
I am planning to use Dexa to induce atrophy in C2C12 for 24, 48 and 72 hours but I am confused whether I should use serum free DMEM for treatment or DMEM with 10% FBS?
Need your recommendation
Hi Muhammad Arif Aslam
For this paper: Article Nilotinib impairs skeletal myogenesis by increasing myoblast...
I used Dexa in differentiation media (2.5% Horse serum in DMEM-High glucose (DMEM, high glucose, GlutaMAX™ Supplement, pyruvate, Gibco 10569010)). Let me know if you have further questions to my email: [email protected]
Hi Osvaldo Contreras
I also have the same problem with Muhammad Arif Aslam
I differentiated C2C12 cells in differentiation medium(DMED+2%FBS+1%PS) for four days and then intend to induce myotube atrophy by treating them with 10 μM DEX for 48 hours.
However, I'm unsure whether to prepare DEX using growth medium or differentiation medium ?
so, according to your experience , do I need to use the differential medium? ( my way to prepare the differential medium is DMED+2%FBS+1%PS. is it correct? )
thanks
Hi Chen wei Chen
I would strongly recommend you preparing DEX in differentiation media. I have been using the following differentiation media (2.5% Horse serum in DMEM-High glucose (DMEM, high glucose, GlutaMAX™ Supplement, pyruvate, Gibco 10569010)).
So, again please use the differentiation medium.
All the best,
Osvaldo
This was a problem I encountered 4 years ago during my PhD, I overcame the problem and was able to use starvation as a model for muscle atrophy.
Article A transcriptomics-based drug repositioning approach to ident...
Hi Osvaldo Contreras
Thank you very much for your advice!
I also want to ask again, if I use DEX to treat myasthenia gravis for 48 hours, do I need to change to a new DEX at 24 hours to prevent the weakening of its effectiveness? (At the same time, I will do some therapy(stimulation) to myotube at 24hr and 48hr aspectly.)
Thanks!
Chen wei Chen I don't think so, but treating these twice may be a better approach to have an even more pronounced effect. I would recommend you test every other day and/or only once treatment. :)
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