Hi,

For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well as the blocking step (1 hour @ 25°C with 10% NDS). I have repeated the staining protocol several times and the bakground difference is the same time and time again. It is also consistent no matter what part of the brain or brain stem I look at. How to approach this difference? Is this definitely non-specific staining? What do you suggest?

Thanks,

Jan

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