If i want to test a particular compound/metabolite on my yeast strains ?

If i want to test a particular compound/metabolite on my yeast strains ( which have been transformed with the desired disease plasmid and shows the phenotype) . Then what is the method that I...

06 July 2018 3,077 0 View

Area under disease progress curve?

In plant pathology experiments generally disease severity measured in terms of grades of affected leaf area in relation to time is used for plotting the area under the disease progress...

05 June 2018 7,948 0 View

Which is better to suspend extracted DNA?

Which is a better one to suspend extracted DNA? TE buffer or ultrapure water?

05 June 2018 3,821 4 View

Long term storage of leaf tissue in CTAB buffer?

Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube...

05 June 2018 1,556 1 View

CTAB DNA extraction method for insects?

IS CTAB buffer method of DNA extraction suitable for extracting insect DNA?

05 June 2018 7,925 2 View

Avoiding clumping of DNA ladder?

my 100 bp and 1kb ladder are not separating properly in gel inspite of providing the optimum agarose concentration, voltage and running time. the individual size bands dont spatially separate...

05 June 2018 1,059 0 View

Pre heating of CTAB buffer?

In what way does preheating of CTAB buffer prior to DNA extraction from leaf tissues improve the efficacy of DNA extraction or DNA recovery

05 June 2018 3,579 4 View

Preserving clones in glycerol stock?

I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol...

04 May 2018 3,469 2 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

03 April 2018 1,479 3 View

Readymix of mastermix and long term storage?

Is it possible to make a readymix of PCR reaction cocktail involving components like water, dntps, taq polymerase etc. and keep it under long term storage for future use or should we use the mix...

03 April 2018 9,805 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View