09 September 2011 2 3K Report

Hey guys, please explain to me how i would know that a buffer i have prepared is actually working e.g. ctab, TAE or TBE buffer. am from college and just secured a place as a molecular biology lab technician so i really need your help.

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Breaking pupal diapause in saturniids (Emperor moths)?

I am trying to rear saturniid moths in the lab and they keep diapausing at the pupal stage. They stick at this level for months on and am unable to raise a colony. Anyone knows how I can break the...

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Synchronizing mating and breaking diapause in saturniid moths?

We are trying to maintain a colony of saturniid moths collected from the field. However, we are experiencing problems with mating. The males and females emerge at different times. These moths only...

10 November 2016 4,311 4 View

Can I use primers that were used with a taqman probe for qpcr using sybr green?

I want to quantify RNA plant viruses using RT-qPCR. I have some primers that I found in a publication. The primers were used witha taqman probe. However, I have SYBR green mastermix in the lab. Is...

07 August 2015 8,832 5 View

How can I perform plant sample sterilization before RNA extraction?

I am looking for alternative hosts of a maize virus. I want to sterilize the leaf samples of the suspected hosts before total RNA extraction, since I am working with an RNA virus. I just want to...

09 October 2014 1,634 3 View

What does this error message mean and what can I do?

I tried to submit sequences to the GenBank, but got an error message indicating that there are non-IUPAC residues in the sequence. What does this mean and what can I do?

08 September 2014 6,847 3 View

Can someone help with DNA sequencing and analysis?

Am working on a plant RNA virus. I did RT-PCR then purified the PCR products and sent them for sequencing. Note: I did not excise gel. However am not sure whethet the sequences I got are ok. The...

07 August 2014 790 5 View

Any advice for sampling maize and wild grasses for viral RNA extraction?

I want to collect leaves from maize and wild grasses from the field but am not sure what method will be best to maintain the integrity of the viral RNA for extraction and RT-PCR. Should I use FTA...

31 December 2013 2,032 3 View

DNA quantification

I am working with a plant pathogen that cannot be cultured, I want to determine the difference in concentration of the pathogen in different parts of the plant for the purpose of standardizing my...

06 July 2012 2,560 8 View

Quantifying DNA using gel photos

I would like to quantify DNA using gel photos instead of extracting the DNA from the gel. Please help if you have information on a software that I can use to do this.

02 March 2012 6,879 9 View

Negative control turning positive in Loop Mediated Isothermal Amplification of DNA (LAMP).

I am using LAMP to amplify 16SrRNA for detection of phytoplasma in napier grass. In the past week, the negative control has been appearing positive on agarose gel electrophoresis. I even tried...

02 March 2012 457 3 View

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