Why am I seeing a fluctuating GCMS baseline/ baseline noise?

I am working with a 7820A GC and 5977B MS. Recently, we changed the helium tank and since then we have been seeing baseline noise in the chromatogram which is high enough to interfere with my...

11 November 2020 6,520 2 View

Is passage number of cell line crucial for developing leukemia in SCID mice?

Hi everyone, I am trying to develop leukemia in NOD SCID mice with BCR/ABL over expresssed cell line. But somehow the mice is not showing any signs of the disease. The passage number of cell line...

25 October 2020 5,614 3 View

Can anyone suggest some articles on herbal medicines as potential drug against COVID 19?

Molecular approach, Pathway blocking, Efficiency of the drug, How much the research done on it till now, Indian distribution of these plants

22 October 2020 319 9 View

Can the venue of a conference become more important that the subject?

When I looked at the controversy around holding or not holding the World Congress of Anthropology 2023 at the venue of Kalinga Institute of Social Science (KISS) in the Odisha state of India, I...

21 September 2020 2,290 4 View

Can anyone suggest the exact method to calculate Single-Band Effective Mass Hamiltonian for Germanane chair structure?

Hi all, Currently I am working on NEGF methodology. I read the atomistic Hamiltonian calculation method. But I need to know the method to generate single-band effective mass Hamiltonian for...

12 September 2020 5,297 1 View

How to do simulation of Wood polymer composites in ANSYS?

I need a detailed explanation on how to add materials since it has two different materials. And step by step procedure and which module to be used for perfect simulation

07 September 2020 7,885 3 View

How do you dissolve polyethyelene terephthalate (PET)?

I want do dissolve about 225g of PET. So I want to know the quantity of solvent to be used.

03 August 2020 507 3 View

Question on detection of DNA Protein covalent complexes by dot blot. How to achieve reproducubility?

I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...

30 July 2020 949 4 View

Why does the equation of balance of Equilibrated force (equation containing voids) not include any thermal relaxation parameter?

In most mathematical problems considering voids, why does the equation of balance of Equilibrated force not include any thermal relaxation parameter considering Green-Lindsay theory or any higher...

20 July 2020 5,481 6 View

What can these peaks in PL corresponds to ?

In Zno (pristine and doped) kindly help in the analysis of these PL results at exc. 510 and 380

26 May 2020 7,393 7 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View