Bradford's protocol?

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Semi-Supervised Learning in Statistics?

Semi-Supervised Learning in Statistics

15 February 2024 8,619 2 View

Can anyone help me how to design multi-section feed line for microstrip patch antenna?

how the width and length and width calculated for 2 section or three- section feed lines

10 April 2023 5,134 3 View

Sizing PV Panels software?

what is the best open source software for sizing photovoltaic solar panels

24 July 2020 3,998 1 View

Confinement and back to university?

After months of confinement and interruption of student-teacher contact, in your opinion, how should the resumption of face-to-face lessons take place? Do the exam subjects have to take this...

24 July 2020 1,527 14 View

Do you have a dataset for welds needed for artificial Intelligence model?

I am keen to develop an artificial neural network and train it on welding data to predict quality of weld based on the welding parameters.

10 June 2020 1,773 0 View

Experimental data of solar drying process?

The confinement due to the corona prevents me from going to the laboratory to do my experiments. Can you help me by giving me the drying data for the tomato, onion and apple: -drying air...

08 June 2020 9,592 6 View

Which part of FPGA can be used to interfacing the finger print device? For aquisition the data of finger print?

I want to connect the finger print device with FPGA to get real time data for processing.

22 March 2020 3,678 4 View

Can anyone give some advice on how to avoid precipitation in artificial sea water after autoclave ?

I prepare artificial sea water (for bacterial culture) by adding salts into MilliQ water. My problem is that after putting the artificial sea water to autoclave there's a precipitate forming. I am...

14 October 2019 1,101 2 View

how can load my data when we use Scatterplot3d from R grouping?

Please I download R packages for Scatterplot3d through this link http://www.sthda.com/english/wiki/scatterplot, but I can't know how can I load my data?

03 August 2019 7,157 0 View

What the comparison between electron ablation and radiation ablation and what are they?

Ablation is the removal of electrons by laser

12 March 2019 3,605 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View