I am differentiating bone-marrow cells into bone marrow-derived macrophages (BMDM) using M-CSF.
What is a reliable specific gene marker for bone marrow Common Myeloid Progenitors that is lost or downregulated during differentiation into monocyte and then macrophage?
Thanks,
J
I may tell you surface markers to differentiate CMP vs Monocytes vs Macrophages, which may be used for flowcytometry.
For CMP: CD34+, CD38+, CD45RA-, CD61-, CD71-, CD123+
For Monocytes: CD34- CD45+, CD14+, CD33+
For Macrophages: HLADR++, CD80/86
Tie-2 (angiopoietin receptor) is expressed in early bone marrow progenitors that belong to the hemangioblast phenotype. It's lost with differentiation into transient amplifying cells of the different BM lineages. Remains expressed in vascular endothelium. Thanks Jitendra Kumar Shandilya for the list.
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