I'm developing a lectin biosensor. I have optimized all steps of the construction based on the impedance signal (delta Rct = Rct analyte – Rct blank) of one concentration of the analyte.
Now, I'm trying to do one calibration curve.
My biosensor does not distinguish different concentrations of my analyte. I tried to incubate the biosensor with the analyte for 30min and 5min, and I have the solutions more concentrated grouped and the solutions less concentrated grouped too. What do you recommend?
Li-Fan Hsu
Hi Diana
Maybe you should try CV and IT first.
If it is still no dose-dependent response, you can try to change the concentration range or voltage range or environment condition like pH.
I hope this helpful to you.
Regards,
Li-Fan Hsu
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