in our research, we reduce the lag phase of biogas generation(4-6 hours) and reached very early biogas production at high yield in a batch system (HRT=19 hours). how can I identify the microbial variety of inoculum? what do you consider as an important factor responsible for such early and high biogas production (y=460 ml/g vs)?
Dear Javad,
The increase in biogas in early stage depends on the method you applied for reducing the lag phase such as pretreatment. For finding the microbial community you can take samples from early stage and perform High throughput sequencing (HTS) to see abundance at phyla, class and genus level. There may be some increase in hydrolysis stage in your case which can be considered as an important factor for lag phase reduction and high biogas yield.
If you need further help in discussing your results, please contact me. My email id is [email protected]
Regards
Hi Mohammad Javad Bardi
if the inoculum was taken from an active anaerobic digester treating similar waste like the one provided in your reactor lag phase can be reduced. The amount of inoculum added also plays an important role.
There are several ways to study microbial diversity. Like mentioned by Asad Ali Zaidi you can try HTS. In this case you need to extract DNA (e.g. MoBio: https://mobio.com/us/shop/dna-isolation/di-soil/dneasy-96-powersoil-pro-qiacube-ht-kit/). You can check google scholar for 'microbial community analysis in anaerobic digesters' (e.g. Article Microbial Community Analysis of Anaerobic Reactors Treating ...
).In addition you can also consider community-level physiological profiling using Biolog's EcoPlate (https://biolog.com/products-portfolio-overview/microbial-community-analysis-with-ecoplates/). The slightly tricky bit is to maintain anaerobic conditions while diluting and dispersing your sample as well as incubating the EcoPlates.
In addition to the above replies, Biogas is a renewable energy source which has both socio-economic and environmental benefits, unlike fossil energy that is highly polluting. By replacing fossil energy with biogas, the emission of carbon dioxide into the atmosphere is reduced.
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