In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

What is the best quality DNA for SSR fingerprinting?

Nanodrop result of my DNA samples extracted from fresh finger millet leaves showed 260/280 ratio ranged from 1.7-2.2 whereas 260/230 ratio ranged from 1.1-2.2. How can I assess the quality of such...

30 November 2020 8,689 6 View

What open plant and crop remote sensing datasets are known?

What open plant and crop remote sensing datasets are known? Wheat, soybeans, potatoes, rice, barley, cotton, Corn, millet, sorghum, sugarcane, apples, grapes, tomatoes, canola, clover.

11 December 2019 5,106 8 View

Can biotechnology play a leading role in resolving the issues of hidden hunger?

challenging the hidden hunger through biofortification of crops

10 November 2018 5,977 7 View

Why pearl millet wild type has hygromycin gene?

I use hygromycin gene primers to do PCR for ICMB 01222 pearl millet wild type and get a strong band. After sequencing, the sequence is hygromycin gene. I feel very confused. I also use water as a...

06 July 2018 1,269 0 View

Genetic basis of downy mildew resistance in pearl millet?

Genetic/ Molecular basis of downy mildew resistance in pearl millet?

02 March 2018 2,015 3 View

How to produce aflatoxin (secondary metabolite) of Aspergillus flavus?

Hi everybody ... When is the fungus exposed to hard conditions or exposed to typical conditions? Aflatoxins are poisonous carcinogens that are produced by certain molds which grow in soil,...

01 February 2018 9,658 18 View

What are the effects of long term rice cultivation in hot arid environment of India (Rajasthan)?

Arid Ghaggar-flood plains of north western India, which is traditionally not a rice growing region, has started growing during the late 1975 when canal network was brought in this region. As a...

07 August 2017 701 0 View

Can anyone suggest a spectrophotometric assay of lipase activity assay from pearl millet seeds? ].

07 August 2017 9,150 2 View

What scientists are concerned with the question of Synthesis of Nitrogen Nanoparticles and what are the prospects for this problem?

07 August 2017 8,655 12 View

How can I calculate LOD and LOQ in HPLC-MS/MS?

Hi, Dear All, I got some results of my master thesis (atropine and scopolamine detection in contaminated millet with Datura seeds) with HPLC-MS/MS. Now, I want to calculate LOD and LOQ. I know it...

07 February 2017 4,458 3 View