How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What is the best quality DNA for SSR fingerprinting?

Nanodrop result of my DNA samples extracted from fresh finger millet leaves showed 260/280 ratio ranged from 1.7-2.2 whereas 260/230 ratio ranged from 1.1-2.2. How can I assess the quality of such...

30 November 2020 8,689 6 View

What open plant and crop remote sensing datasets are known?

What open plant and crop remote sensing datasets are known? Wheat, soybeans, potatoes, rice, barley, cotton, Corn, millet, sorghum, sugarcane, apples, grapes, tomatoes, canola, clover.

11 December 2019 5,106 8 View

Why pearl millet wild type has hygromycin gene?

I use hygromycin gene primers to do PCR for ICMB 01222 pearl millet wild type and get a strong band. After sequencing, the sequence is hygromycin gene. I feel very confused. I also use water as a...

06 July 2018 1,269 0 View

Genetic basis of downy mildew resistance in pearl millet?

Genetic/ Molecular basis of downy mildew resistance in pearl millet?

02 March 2018 2,015 3 View

How to produce aflatoxin (secondary metabolite) of Aspergillus flavus?

Hi everybody ... When is the fungus exposed to hard conditions or exposed to typical conditions? Aflatoxins are poisonous carcinogens that are produced by certain molds which grow in soil,...

01 February 2018 9,658 18 View

What are the effects of long term rice cultivation in hot arid environment of India (Rajasthan)?

Arid Ghaggar-flood plains of north western India, which is traditionally not a rice growing region, has started growing during the late 1975 when canal network was brought in this region. As a...

07 August 2017 701 0 View

Can anyone suggest a spectrophotometric assay of lipase activity assay from pearl millet seeds? ].

07 August 2017 9,150 2 View

What scientists are concerned with the question of Synthesis of Nitrogen Nanoparticles and what are the prospects for this problem?

07 August 2017 8,655 12 View

How can I calculate LOD and LOQ in HPLC-MS/MS?

Hi, Dear All, I got some results of my master thesis (atropine and scopolamine detection in contaminated millet with Datura seeds) with HPLC-MS/MS. Now, I want to calculate LOD and LOQ. I know it...

07 February 2017 4,458 3 View

How to analyze data from an augmented design

I have conducted trials on downy mildew of pearl millet into 4 locations. The design used was an augmented design in each of the location (I did not have enough seeds). I would like to know how to...

09 October 2016 1,091 9 View