If a particular bacteria's biofilm mature at 48 hr and we are trying to study its disruption using particular treatment
. We give the treatment at biofilm maturation i.e 48 hr. And then quantify disruption after 24 hours of treatment. which culture control should we consider the one at biofilm maturation i.e 48 hr or the other after 24 hr after the treatment i.e 72 hr. what i observe in tissue culture plates at 72 hr the biofilm goes in declining phase, that jeopardize the disruption result if we consider the control at 72 hr. Please suggest me which control consideration is correct?
Not sure if I fully get you, but the gist i got, I would make the 72 hours treated the experiment and the 48 hours untreated the control group since its the baseline from which you are basing on
Hello Priyanka! In my personal work I take the 48h control group as the baseline since it is facing the same treatment as the ones that you are testing. As you point out, the 72h group is too old and the biofilm produced will redeem the quality and won't be a good control.
Sending you best regards,
Carla.
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