Santos Alonso

3 Questions 13 Answers 0 Followers

Questions related from Santos Alonso

Is dsDNA Shearase a good alternative to sonication in MeDIP-Seq?

Hi everyone, I am trying to do some methylation analysis (human DNA, NGS) and I've come across several alternatives. All of them need to fragmentate the input DNA. Some make use of sonication...

04 January 2018 4,530 1 View

Is enzyme fragmentation a good alternative to sonication in ChIP-Seq?

I've read that an alternative to sonication is fragmentation by MNase. But people seem to have different opinions on the benefits or inconveniences of using one or the other. Can some one give me...

28 October 2016 5,830 2 View

Which type of sonicator is best for Chip-Seq?

For ChIP-Seq analysis I've seen people describing water-bath sonicators, probe-sonicator, sonicators in the Hz range, in the kHz range...different W... Can anyone help in explaining which (and...

28 October 2016 8,048 8 View