Determination of partial specific volume?

Dear all, For some applications in AUC, we would like to correctly determine the partial specific volume of our biomolecules. We currently use Sednterp as a principal approach which diplays a...

06 July 2019 5,392 1 View

What condition should I use for analyzing intact protein on a MALDI Mass Spectrometer?

Hello, I am working on a biophysics core facility where we have developed a service to check among other things the molecular mass of the protein of interest, the presence of contaminants and...

04 May 2019 5,435 17 View

Multiple charges in MALDI-TOF?

Hello, Do you have a robust method to get multiple charges for proteins in MALDI-TOF? I would like to improve the precision on the mass for big proteins. I work with a UltraFleXtreme from...

04 May 2018 8,361 2 View

Why DHB is a reducing agent while sDHB matrix is not a reducing agent?

Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...

09 October 2017 6,560 4 View

Range of second virial coefficient

Hello, I would like to work with the second virial coefficient (A2 or B22) to determine the best conditions to formulate protein buffers and also to determine the best conditions for...

07 August 2017 8,980 3 View

Preparation of MALDI TOF experiment?

Hello everyone, I am new in the field of mass spectrometry and I have some questions about the preparation of the matrix and analyte for a MALDI TOF analysis. I prepared a saturated solution of...

09 October 2016 9,567 4 View

Refractive index increment of amoxicillin?

01 January 1970 2,059 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View