I want to do knock out of SHV class of beta-lactamase from Klebsiella pneumoniae genome as a part of my PhD work. I am confused with the techniques available. I have never done knockout before. Your suggestions will be highly appreciated.
Ajeet Singh
u can use recombination method, in that u take nearby 50-70 nts upstream part of your gene and same downstream and fuse with reporter gene and do transformation. for more information, u can call me dude.
1 votes
0 thanks
Gaurav Kumar
Thanx alot, I am using one step deletion strategy that includes recombination of homologous sequences.
@Milind- your suggestion will take me no where. You idea holds good for a random mutation but not for a specific gene deletion. Thanx alot for your idea.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques
More Gaurav Kumar's questions See All
Similar questions and discussions