13 Questions 27 Answers 0 Followers
Questions related from Ajeet Singh
I am working on bacterial protein, which will be crystallized for structural study. My protein MW is 22kDa and pI is 9.2. I have tried crystallization trial in 20mM Tris, 150mM KCl and 5 %...
25 November 2014 2,698 4 View
I am working one bacterial protein pI 4.5, and want to screen optimum stability condition through Differential scanning fluorimetry (DSF). I am new to DSF and don't have much experimental...
26 August 2014 9,588 3 View
I am working on bacterial protein crystallization, and has been purified in 20mM Tris buffer(pH 7.5), 200mM KCl and Glycerol 10%. I have optimized at small level that if glycerol is completely...
23 August 2014 2,289 9 View
I am working on bacterial protein crystallization (protein pI 9.2). During purification, I usually maintain 200mM KCl salt (20 mM Tris 7.5, 10% glycerol) in final elution buffer and followed by...
16 August 2014 6,283 13 View
Based on In Silico analysis, I have found that my protein is having metal binding properties. I want to confirm it using experimental method like binding site is there or not and which metal is...
21 July 2014 7,233 2 View
One of my research protein is bind to phospholipids, for elucidation of this binding mechanism I am trying to make lipid micelle for my interaction study through ITC. I am making stock of...
12 July 2014 8,097 3 View
I am working with a protein which make bacteria resistant against organic solvents like toluene or benzene. Recently it was reported that this protein also has a binding affinity with...
04 June 2014 1,264 1 View
My expressed protein is being collected in the pellet fraction and I have used different strategies for making it soluble but still its coming in the pellet. I have used strategies like...
11 February 2014 898 31 View
I purified my recombinant His tag protein in Tris, pH 7.5 (pI of protein 8.9) using 400mM Imidazole conc. in elution buffer. I dialysed my protein for removing imidazole but it is being...
04 October 2013 9,823 21 View
I am getting non specific PCR, I have tried with increase temperature and decreasing primer concentration but still non specificity is there? What parameters should I consider now?
01 October 2013 3,368 12 View
Many people prefer fresh competent cells for protein purification of their crystal structure. Is it really true that using fresh competent cells gives a better chance of getting protein crystals?
05 February 2013 2,016 5 View
I have tried tim17 (membrane protein) with strain C-41, Rosetta, BL-21 and different temperatures. Is there any other parameter that I should try for tim17 induction?
23 January 2013 9,779 2 View
I have tried to purify membrane protein in HEPES buffer but still there are some other buffers there. Which buffer and other biochemical parameters should be followed for better protein induction...
16 January 2013 1,804 5 View
