Somatic mutations - how to validate NGS data?

Hi, I'm searching for methods to validate somatic mutations in pancreatic beta cells. The mutations are found by Next Generation Sequencing and I have try Sanger Sequencing with no results.

12 March 2018 1,821 5 View

Glucose stimulation of rat beta cells for insulin measurement?

Can anyone suggest a protocol for glucose stimulation of rat beta cells for insulin measurement. I can see final glucose concentration as 16,7mM but I dont know why?

26 February 2018 4,742 3 View

Best way to inactivate RNase III-A?

I require 100 uL of bovine ribonuclease III-A stock solution (5mg/mL). The product from Sigma-Aldrich is a very fluffy substance. I need to inactivate this twice at 100 degree C twice. Does anyone...

31 January 2017 1,632 3 View

Normalization of small RNA-seq with housekeeping RNA?

I am working on some small RNA-Seq data and trying to normalize them. From literature, I found that people are using some housekeeping small RNA as reference for the normalization in qPCR. Can...

01 November 2016 4,047 3 View

What is production mechanism of Haft Kel field?

how is Haft Kel Field produced and how is the CO2 as injection considered in the flow simulation?

09 July 2016 882 2 View

Shelf life of TRIS?

I just started in a new lab and am about to start running western blot. However, the TRIS powder they have here is from 2002 and I'm a bit reluctant using it. Does anyone have experience using old...

26 April 2016 6,451 5 View

Does anyone know of an efficient, low-toxic caspase-1 inhibitor for in vitro cell culture?

I would like to inhibit the inflammasome in my cultured dendritic cells, but am a bit unsure of which one to buy. Does anyone have any recommendations? Thanks!

18 August 2013 2,847 2 View

Two questions: using fibronectin in transwell migration assays?

I've been having a lot of trouble trying to get my assays to work. I'm concerned that all I'm seeing is background migration. It's just B16BL6 melanoma cells (usually 1 x 10^5), using 10% FBS as a...

14 May 2013 5,684 15 View

Is possible to publish a phage draft genome as announcement in a journal (e.g. J virol or J Bact) or must you have the complete sequence?

What is the minimal genomic information to required to be accepted for publishing?

21 March 2012 4,643 1 View

Stats to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after resto??

Hello all, I am looking for ways to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after restoration? My data set contains a...

08 February 2021 7,887 5 View

What is the best geophysical machine or device for detecting groundwater?

what is the best geophysical machine or device for detecting groundwater? Are there any machine that can detect the depth and the type of water? do I have to buy one machine or more than one to...

26 January 2021 6,149 15 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

Is there any low-cost filtering system (or other tech.)to lower the salt conc. (salinity) of surface/groundwater for large scale agricultural system??

The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...

18 January 2021 5,015 3 View

Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View

Please, any real time simulation tool recommendation for islanding detection and control in DG to me aside MATLAB as it keeps freezing and very slow?

I am currently working on islanding detection and control in distributed generations. I would kindly ask that you recommend a real-time simulation tool that helps me in this study. I have tried...

13 January 2021 3,626 3 View

Could you help me to find a research topic or something related to the Islanding detection on smart grid system?

06 January 2021 6,508 5 View

How to estimate the ground water contribution while calculating the water use efficiency of a crop?

Ground water contribution is important while estimating the water use efficiency for a crop for calculation of ET without the use of Lysimeter

22 December 2020 1,254 2 View

Can anyone kindly explain what is the best method to determine the statistical correlation between two raster maps in same pixel size?

I need a method to determine statistical correlation between two GIS based raster maps. Eg. Correlation between Soil Erosion rate map (Raster) and spatial distribution map of any groundwater...

04 December 2020 9,920 11 View

Can we assume partially saturated soil conditions during Vacuum Consolidation Method?

Normally we assume that the zone of vacuum consolidation is fully saturated. But I need to find out what effect the existing groundwater table has on effectiveness of Vacuum consolidation method....

30 November 2020 2,978 1 View