Best vector for making shRNA from 21 bp siRNA?

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Is anyone aware of a validated measure for studying workplace motivation?

Specifically what I am seeking to study is those in supervisory roles motivations as it relates to their subordinates. If you are aware please share the measure and related literature associated...

26 May 2018 1,425 2 View

I am seeking answers and literatures for the following questions?

Are there any studies and literature on emotional regulation/labor, dimensions of deep acting and surface acting relationship to individuals’ attitudes? Attitudes such as hiring decision,...

04 March 2017 3,959 1 View

Is there a validated survey instrument for measuring organizations bottomline?

Bottomline is a broadly defined term. I an seeking to examine and determine relationships between individuals emotion regulation perceptions and an organizations bottomline. I would like to...

28 January 2017 2,034 2 View

What is the impact of how to regulate work emotions net affective on organizations profits?

I am seeking relevant literature and testing methodology for a concept. a. Does one see their selves – more or less as a leader able to regulate work emotions? i. Test the...

28 January 2017 3,490 2 View

Can organizational support theory be measured?

Organizational support theory, assumes employees form belief regarding their organizational values, their contributions and organizations cares about their well-being. o Can this theory be...

25 June 2016 9,846 8 View

I am seeking literature and/or any knowledge regarding emotional intelligence influencing investment decisions?

Is there an impact when corporate data is utilized are emotional intelligence individuals more reliant on this data? Is there a difference in investment decision from emotional intelligent than...

11 March 2016 4,967 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View