Studying bacterial lipids extracted using Bligh-Dyer. Analytes of interest are the phospholipids, specifically PE and PG (these dominate in E. Coli, for example). Using LC-MS (ESI) for analysis. What is best sample diluent to re-dissolve the final dry-downs from the Bligh-Dyer?
Note that my LC gradient starts at 15% Aq and 85% MeOH, so need to be (at least somewhat) mindful of the whole "don't dissolve in diluent that is stronger than your initial gradient" idea.
Thanks!
I would think that your best bet would be to dissolve in a (relatively) small ammount of 100% organic solvent (MeOH or even a mixture of MeOH and choloroform) and then dilute to 50% MeOH:Water afterwards. Less than 50% organic in the final dilution and you might start to see precipitation while dissolving in such high water content from the start will make it hard to dissolve the whole pellet and you might get very high %RSD on recovery in that case.
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