Is grain boundary scattering 'always' dominant over surface scattering in grained metal/semiconductor thin films?

Hi all, I was wondering if there is some theoretical insight in the relative contribution to the resistivity of grained thin metal or semiconductor films coming from bulk grain boundary...

18 December 2020 2,969 3 View

Can I use g_MMPBSA with Cas9?

Hello, I'm trying to see the interaction between the Sa Cas9 protein and the guide RNA. I ran a molecular dynamic simulation on it and then estimated the energy binding using the g_MMPBSA...

17 December 2020 661 2 View

What is the Method of Moment theory and what differentiates it from the MLFMM solver?

Hi everyone, It's been quite a time since I have started searching the web for clear explanations on the Method of Moment (MoM) theory for electromagnetic field computing. Does anyone know a...

23 September 2020 2,763 4 View

What are the latest research in control system ?

Hello all, I want to do my research in control systems (nonlinear, robust, adaptive) and I'm wondering what to study ? Do you guys have any ideas ? I am a bit lost as those fields are...

06 February 2020 8,659 15 View

How to make multivariate time series regression in R?

I m analysing the determinant of economic growth by using time series data. Collected data covers the period from 1980 to 2017. There is one dependent variable and 12 independent variables. The...

06 January 2020 8,411 14 View

Can the bending of a light ray passing the sun correctly be explained by gravitoelectromagnetism?

04 October 2019 9,860 9 View

How to Conduct SPSS Reliability Test For Multi-variable Questionnaire?

Greetings, I am a DBA student conducting a study about "Factors Influencing the Adoption of Payment Cards in Lebanon". My research model, theoretically based on the Technology Adoption Model...

29 January 2019 2,369 7 View

Issues with diagram in AMOS, can anyone help ?

Hello, I'm a student and for my last essay, I have to use structural equation modeling. I'm kind of lost here, and I don't really know what i'm doing on the software, and I have poor statistical...

14 August 2018 8,002 3 View

Has anybody an experience of Fluid exchange interaction into Abaqus CAE Finite Element Package ?

Hello everyone, I am investigating Pressure evolution in response to external solicitation in Finite Element Analysis. I try to iterate my model by enabling a Fluid Exchange interaction between...

14 November 2017 6,973 5 View

How to compute the ionization energy within a frozen solvent cage in PCM

Hi, I'm trying to calculate the IE of a molecule (solute) within a solvent. It seems like I can get a fair approximation using PCM solvation model, however I do not want to let the environment...

30 July 2017 8,247 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View

What is the optimal propagation regime for pET-1M(KanR) and pMCSG7(AmpR) plasmids in DH5alpha E.coli?

Dear all, I am trying to harvest the low-expression plasmids in the mentioned E.coli strain. I constantly gel very low concentrations (30-50 ng/uL while the concentration is usually around 100...

08 February 2021 4,255 4 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Can I RNase A treatment my extracted plamid?

I have extracted the plasmid with the Qiagen maxi Endofree kit. the result of gel electrophoresis for this sample showed a band of degraded RNAs. Now, I want to remove RNAs from the eluted plasmid...

25 January 2021 2,958 3 View