Hi all,
I would like to know what you consider as the best resources for cell culturing and tissue modeling for a newbie?
Thanks so much
Sonja hello,
You can start with these:
www.springer.com/gp/book/9780306458590
www.amazon.com/Tissue-Culture-Techniques-Bernice-Martin/dp/0817637184/ref=sr_1_8?keywords=cell+culture+histology&qid=1570552971&s=books&sr=1-8
www.amazon.com/Introduction-Cell-Tissue-Culture-Introductory/dp/0306458594/ref=sr_1_3?keywords=cell+culture+histology&qid=1570552971&s=books&sr=1-3
www.elsevier.com/books/contamination-in-tissue-culture/fogh/978-0-12-261850-5
www.springer.com/gp/book/9781588292841
Also, check out these handbooks:
https://www.vanderbilt.edu/viibre/CellCultureBasicsEU.pdf
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/General_Information/1/fundamental-techniques-in-cell-culture.pdf
I hope this helps :)
Hi there,
We source our cell culturing flasks and stripets from Corning.
Our media comes from either sigma-aldrich or gibco. Special media can come from elsewhere, such as lonza.
Our cell lines mainly come from ATCC.
As a general rule sigma-aldrich has most of what you need for day-to-day culturing.
That's pretty broad.
Cell culture... primary or cell lines? Cell lines you can find in ATCC but primary cell protocols depends on the population of interest.
Tissue models...2D or 3D models or computer models? And what type of tissue?
Good day, Please could you help me identify these spores found in wheat roots? The foliar symptoms excipt that of Soil-Borne Wheat mosaic Virus hence I was looking for Polymyxa graminis but...
12 October 2023 2,937 1 View
I recieved EMP data from zoned amphibole, clinopyroxene and plagioclase from my rock sample. It doesn't contain garnet, only few biotite, some quartz and carbonate minerals. In my opinion...
08 February 2022 5,812 0 View
Hi everyone, I am currently trying to establish an assay based on the oxidation of isolated human mitochondrial DNA. I have been trying to isolate the mtDNA myself, and oxidize it afterwards,...
03 November 2021 5,937 0 View
During Seahorse mitostress assays, we have great variance between different cell lines regarding their oxygen consumption. One cell line is tricky because at relevant confluency, the basal OCR is...
23 August 2021 3,017 2 View
Goo day; Wheat samples were bought into the lab with yellow stripes and mosaic-like symptoms on the leaves. The diseased leaves were sterilized with 1% NaOH and plated out on pda-C. plates were...
22 August 2021 1,810 9 View
Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...
06 January 2021 448 4 View
Good day, please assist me with the following. what are these Round structures found inside Wheat roots? Roots were in KOH Solution overnight and autoclaved for half an hour. Then viewed und a...
10 August 2020 9,039 3 View
I am interested in comparing the protein:lipid of pollen between three sister plant species. This information will then be linked to pollinator communities and rate of visitation to each of the...
16 May 2020 7,418 0 View
I performed multiple linear regression, and for my report I need the 95% CI of the standardized bèta's. However, SPSS only gives the 95% CI for the unstandardized B. How to obtain the CI for...
28 April 2020 2,638 3 View
Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...
18 March 2020 5,225 16 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View