Hi all,

I'm trying to develop an ADCC assay for my neuroblastoma lab. I've not done them before and I'm having a tough time finding anyone where I work who has. I have a couple of questions that hopefully someone can answer.

1) What is the optimal source of effector cells? A cell line, or PBMCs? We are primarily interested in NK cells for this- are NK92 cells appropriate, or do I need to find specifically CD16+ NK92 cells? Are any other cell lines better specifically for ADCC?

2) I'm assuming a negative control is simply an isotype control. Is there a need for a positive control? If so, what?

3) When you do these assays, do you report CD107 on the NK cells and the % dead of the target cell? I'm planning to use flow cytometry, not radioactivity.

Thanks for any suggestions!

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