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Questions related from Michelle G Pitts
Everyone knows that LPS is pretty artificial, especially if we're talking M1 macrophages for a cancer setting. I've heard something about using TNFa instead of LPS, but darn if I can find it. Does...
18 May 2023 9,376 2 View
I'm new to the field, in a new lab, and looking for macrophage lines that would be best to use for some in vitro work involving adding tumor cells grown/ modified in vitro to macrophages. There...
02 December 2022 9,822 2 View
I started in a lab fairly recently that uses lots of blood/serum for assays. When each study is terminated, the mice are given a lethal dose of xylazine and ketamine and then opened up for...
27 June 2019 8,105 0 View
Hi all! I'm looking to decrease bacterial uptake by PMNs and I've been using Cytochalasin D with limited success. The bacteria are opsonized, so as far as I know this is driving a CR3-mediated...
03 July 2017 4,311 2 View
If I resuspend dihydrorhodamine 123 in EtOH, is it best to store aliquots in -20 or in liquid N2? Also I've seen one recommendation not to use a reconstituted aliquot beyond 1 month. What's the...
09 November 2016 1,004 3 View
This is from bone marrow after a gradient to deplete non-PMNs. I attached a scatter plot showing a group in the upper right accounting for about 6% of my total. These cells are CD11b+, Ly6G very...
18 August 2016 4,094 10 View
I'm planning to isolate BM PMNs and use normal mouse serum to opsonize Listeria. I see in older papers with other bacteria that HBSS with gelatin has been used. I'm not readily finding a reason...
28 July 2016 2,907 3 View
Biorender's prices have gone nuts. Is anyone using an alternative?
01 January 1970 4,779 0 View
