Best p62 Antibody?

More Jonas Walter's questions See All

What is the best Nurr1 antibody to use?

We thought about buying this one: http://www.rndsystems.com/Products/PP-N1404-00 It is used by the Studer Lab. Do you have any other suggestions we could try?

09 October 2014 8,750 2 View

Can you recommend a Lysosomal antibody that is working in human cells, and if possible in PFA fixation?

I am facing the problem that my Lysosomal antibodies, both theoretically detecting Lamp1, seem to be unspecific in my human cells. I verified this by using a Lysotracker. Now I am looking for an...

08 September 2014 2,275 7 View

Can anyone help with confirming R1441G-mutation in an artificial mouse model via sequencing?

I have a strange problem. I am using this mouse model: http://jaxmice.jax.org/strain/009604.html I designed primers to amplify the mutation site (gDNA). This works fine, the primers are designed...

02 March 2014 5,357 5 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

How to measure the concentration of monoclonal antibodieswithout nanodrop ?

Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...

14 July 2024 3,106 3 View

Thomas Gawriluk

The Wako 018-22141 has been used in several papers. For IHC on mouse ovary it has worked great for me.

Regina Mark

anti-p62 / SQSTM1, N terminus guinea pig polyclonal:

http://www.progen.de/en/catalog/product/view/id/1985/s/p62-protein-n-terminus/category/22/?___from_store=de

anti-p62 / SQSTM1, C terminus guinea pig polyclonal:

http://www.progen.de/en/catalog/product/view/id/1984/s/p62-protein-c-terminus/category/22/?___from_store=de

both should work fine for your purpose.