We wish to create an APTES monolayer within a channel. We are currently using 10% APTES in Ethanol, for 10 minutes incubation followed by washing. We have also tried 2% APTES for 1 hour. Both have given inconsistent results.
Could you suggest what would be a good method to achieve more repeatable performance?
P.S. before APTES we are activating the surface using H2O2 with HCL & H2O to generate reactive OH group on surface.
Try to use fresh (it is important) water solution (1%). Surprisingly, it gives the finest monolayer (not sure about PDMS, but it works very well for Si and mica).
There is a reference which provides how to do it.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423308/
Denis, thanks for your answer. How long should we keep it and at what temperature?
Rohan, no worries. For Si or mica 10 min at room temperature was enough.
Thanks Denis Korneev . I had read that water with APTES can be detrimental as it hydrolyzes APTES. Yet many including you, have suggested using APTES in water or diluted ethanol solutions. What is the reason behind this observation?
Rohan, the exact mechanism is not self-evident, but the effect was shown experimentally almost 30 years ago - water solution gives the finest APTES monolayer:
Article Structure of 3-Aminopropyl Triethoxy Silane on Slicon Oxide
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Graphs