26 Questions 23 Answers 0 Followers
Questions related from Rohan Aggarwal
I am doing an experiment wherein we are depositing some hydrophilic nano-particles on a functionalised surface. We are then displacing the aqueous phase with a high viscosity oil phase. However,...
05 December 2024 2,416 2 View
We have a batch which has partially reduced and smells like ketone. Is it possible to oxidize it back easily. Size is 5um, material is polystyrene.
23 March 2024 9,299 0 View
We use 990 ul of antibody solution and then add 0.4mg EDC and 1.1.mg sulfo-NHS (in MES) and react for 15 minutes at room temperature, before adding it to the amine surface for immobilization. Now...
03 June 2023 1,249 1 View
Hi, We require an automated particle counting system for a particle counting assay we have developed for our lab. The only need is to be able to count absolute number of particles in a solution of...
02 December 2021 4,280 4 View
We are looking for a biocompatible immiscible liquid (with water) with low viscosity & surface tension to enable flow in a microchannel system. Most such liquid tend to have some limited mixing at...
12 February 2021 3,275 2 View
We use a primary amine surface to bind our antibodies. Thereafter we use BSA and other protein blockers for blocking. The results are not really great, and vary quite a bit on repeats. We wanted...
27 April 2020 3,423 1 View
Electrochemical sensors have an inherent advantage for being simple, cheap (glucometers), especially for nucleic acid assays. Yet there are very few health devices in the market for point of care...
23 December 2019 2,823 3 View
We wish to know if it is possible to create a commercial measuring product with nanoliter precision. We are creating a microfluidic device in PMMA. We wish to measure 100 ul volume of a solution...
31 October 2019 7,427 3 View
Hi, One of our silicon masters was accidentally broken while casting in pdms. Getting replacement is not an option at this time. Could you please suggest a way in which we can fix the master such...
21 May 2019 6,015 2 View
We wish to create an APTES monolayer within a channel. We are currently using 10% APTES in Ethanol, for 10 minutes incubation followed by washing. We have also tried 2% APTES for 1 hour. Both have...
07 May 2019 302 6 View
We do not have a system for oxygen plasma, corona discharge or ozone in our lab. We wanted to create reactive surface for our immunoassay on PDMS, such that it can bind to APTES. We have been...
29 April 2019 9,863 3 View
We wish to deposit a surface of a closed microchannel with microparticles (polystyrene) in such a way that it deposits there but do not bind. We will dry the channel post the deposition process....
29 March 2019 9,141 0 View
We are optimizing the assay parameters for our TSH immunoassay. We had been using a 7.4 pH PBS buffer for antibody coating so far, but now want to evaluate if changing the pH of the coating buffer...
30 July 2018 1,321 2 View
The first method is silanization of PDMS surface with 50% v/v in ethanol followed by treatment with Glutaraldehyde which presents a site for antibody binding. In the second method BSA is passively...
05 April 2018 518 4 View
We wish to perform some steady state simulations for our microfluidics related work. We are planning to buy a desktop system to do so rather than do it on cloud, due to some internal issues. But I...
18 November 2017 8,172 3 View
I am trying to fabricate simple microfluidic devices using common laser printer transparencies (polyester based). I will cut my channels using a CO2 laser, and then cover the same with 3mm acrylic...
22 September 2017 684 10 View
We would like to know what is the best option for creating wax patterns on different types of papers, for microfluidic applications. Most literature has quoted inkjet printers modified for wax...
27 July 2017 679 6 View
There are a number of products available in the market from companies such elveflow, fluigent, dolomite etc. Which among them performs best and is worth the price? We are interested in developing...
07 June 2017 599 6 View
I had used a closed micro-channel (0.5 mm height, 5 mm wide) for an immunological reaction. I wish to re-use the same for some minor experiments, and need to remove a small layer of the acrylic...
25 April 2017 3,306 3 View
I wish to import antibodies directly from a manufacturer in Finland for research. They will send it via Fedex. But I am confused if I need a drug import license for this, to get it past customs?...
16 February 2017 5,332 0 View
We wish to find the conc. of our antibody in a solution which is often low (2-6 ug/ml). UV Spectrometry does not work well, since we need to dilute it and need to know the extinction coefficient...
11 February 2017 6,445 7 View
We want to place a large order, but we are not sure how reliable they are. Our local suppliers costs us a lot more, since they also source it from similar foreign merchants, and we wanted to cut...
24 January 2017 9,232 2 View
There are varying views on the choice for capture and detection Ab for immunoassay application. While a large no of people (mostly academic researchers) prefer polyclonal for capture, there is a...
19 January 2017 7,278 6 View
I read that with syringe pumps, the microspheres that I am using may settle down overtime in the syringe. I require flow rates in the range from 0.1 ul/min to around 10 ul/min for my studies. I am...
04 January 2017 2,585 10 View
Is hemocytometer a reliable way to measure the number of microspheres in a solution? I heard flow cytometers provide the best option but they can only count fluorescent microspheres and not...
03 January 2017 1,057 4 View
I performed passive adsorption of protein on my PS microspheres, but there is some minor visible clumping in the final solution. I had performed all steps as per a previously used protocol that...
07 November 2016 7,442 2 View
